Loop Assembly: a simple and open system for recursive fabrication of DNA circuits

Publishing date: 2018-01-18

Published on: bioRxiv

summary: High efficiency methods for DNA assembly are based on sequence overlap between fragments or Type IIS restriction endonuclease cleavage and ligation. These have enabled routine assembly of synthetic DNAs of increased size and complexity. However, these techniques require customisation, elaborate vector sets and serial manipulations for the different stages of assembly. Bernardo Pollak and colleagues here present Loop assembly, based on a recursive approach to DNA fabrication.

authors: Bernardo Pollak, Ariel Cerda, Mihails Delmans, Simón Álamos, Tomás Moyano, Anthony West, Rodrigo A Gutiérrez, Nicola Patron, Fernán Federici, Jim Haseloff

link to paper: https://www.biorxiv.org/content/early/2018/01/18/247593

Icons made by catkuro from www.flaticon.com