uORF-Tools – Workflow for the determination of translation-regulatory upstream open reading frames

Publishing date: 2019-09-26

Published on: PLOS ONE

summary: Ribosome profiling (ribo-seq) provides a means to analyze active translation by determining ribosome occupancy in a transcriptome-wide manner. The vast majority of ribosome protected fragments (RPFs) resides within the protein-coding sequence of mRNAs. However, commonly reads are also found within the transcript leader sequence (TLS) (aka 5’ untranslated region) preceding the main open reading frame (ORF), indicating the translation of regulatory upstream ORFs (uORFs). Here, the authors present a workflow for the identification of translation-regulatory uORFs.

authors: Anica Scholz, Florian Eggenhofer, Rick Gelhausen, Björn Grüning, Kathi Zarnack, Bernhard Brüne, Rolf Backofen, Tobias Schmid

link to paper: 10.1371/journal.pone.0222459

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