Viridot: An automated virus plaque (immunofocus) counter for the measurement of serological neutralizing responses with application to dengue virus

Publishing date: 2018-12-10

Published on: PLOS NEGLECTED TROPICAL DISEASES

summary: Although the plaque reduction neutralization test (PRNT) is an important assay for measuring neutralizing antibody responses against many viruses, no free, open-source programs specifically designed for virus plaque counting and neutralizing antibody titer estimation are available. As described here, Katzelnick et al have developed Viridot, a package for R with a user-interface in shiny, which is designed for use by laboratory-based virologists and researchers with minimal coding experience. The program includes: automatic and user-specification of settings for plaque counting; saving of plaque counting settings; counting of many plates at once; and easy output of plaque counts, plaque sizes, and images with counted plaques circled.

authors: Leah C. Katzelnick, Ana Coello Escoto, Benjamin D. McElvany, Christian Chávez, Henrik Salje, Wensheng Luo,Isabel Rodriguez-Barraquer,Richard Jarman,Anna P. Durbin, Sean A. Diehl, Derek J. Smith, Stephen S. Whitehead, Derek A. T. Cummings

link to paper: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0006862

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